Chemoenzymatic iterative synthesis of difficult linkages of oligosaccharides on soluble polymeric supports

  • Yan F
  • Gilbert M
  • Wakarchuk W
 et al. 
  • 7

    Readers

    Mendeley users who have this article in their library.
  • 20

    Citations

    Citations of this article.

Abstract

A trisaccharide donor containing a cis-Galpα(1?4)Galp linkage was prepared using a synthetic strategy based on chemoenzymatic oligosaccharide synthesis on a soluble polymeric support. Significantly, only retaining glycosyltransferases gave complete reactions, whereas inverting enzymes showed little or no activity with poly(ethylene glycol) (MPEG)-bound lactose as an acceptor. The MPEG-attached trisaccharide was shown to bind to Verotoxin-1 by transfer NOE studies through the Galpα(1?4)Galp portion of the molecule.
A trisaccharide donor containing a cis-Galpα(1?4)Galp linkage was prepared using a synthetic strategy based on chemoenzymatic oligosaccharide synthesis on a soluble polymeric support. Significantly, only retaining glycosyltransferases gave complete reactions, whereas inverting enzymes showed little or no activity with poly(ethylene glycol) (MPEG)-bound lactose as an acceptor. The MPEG-attached trisaccharide was shown to bind to Verotoxin-1 by transfer NOE studies through the Galpα(1?4)Galp portion of the molecule.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Fengyang Yan

  • Michel Gilbert

  • Warren W. Wakarchuk

  • Jean Robert Brisson

  • Dennis M. Whitfield

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free