Cholesterol in remnant-like lipoproteins in human serum using monoclonal anti apo B-100 and anti apo A-I immunoaffinity mixed gels

  • Nakajima K
  • Saito T
  • Tamura A
 et al. 
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We have developed a simple, rapid assay method for apo E-rich lipoproteins (d < 1.006 g/ml), using an immunoaffinity gel mixture of anti apo B-100 and apo A-I antibodies coupled to Sepharose 4B. The immunoaffinity mixed gels adsorb normal lipoproteins containing apo A-I quantitatively as well as most lipoproteins containing apo B-100. Unbound lipoproteins are quantified by assay of cholesterol. Characterization of the unbound lipoproteins of d < 1.006 g/ml (J Lipid Res 1992;33:369-380) has shown that they represent chylomicron and VLDL remnant-like particles (RLP). RLP-Cholesterol(C) levels in plasma have been determined in 363 male and female normolipidemic subjects(mean ± S.D.: 72 ± 16 mg/l) and have been found to be higher in patients with coronary heart disease and familial dysbetalipoproteinemia. Triglyceride-rich lipoproteins may well contain both atherogenic and non-atherogenic particles that can be separated by this simple immunoadsorption assay. © 1993.

Author-supplied keywords

  • Chylomicron remnant
  • Coronary heart disease
  • Familial dysbetalipoproteinemia
  • RLP-cholesterol
  • Remnant-like particles (RLP)
  • VLPL remnant

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  • Katsuyuki Nakajima

  • Toshimitsu Saito

  • Aki Tamura

  • Motoko Suzuki

  • Takamitsu Nakano

  • Masakazu Adachi

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