Thirty chromosome number determinations of ferns collected in southern Ontario are given for 28 species, one form and one hybrid. Of the 19 genera of the Polypodiaceae described in Gray's Manual, 16 genera and 25 species were studied by this author. Interspecific polyploidy was demonstrated in Botrychium, Cystopteris, Dryopteris, and Asplenium. Intraspecific euploidy is present in Asplenium trichomanes, Polypodium virginianum and Phyllitis scolopendrium. Phyllitis scolopendrium var. americana is tetraploid in comparison to the European variety. Fourteen species studied by this author had identical chromosome numbers with the same species studied by Manton (1950), although five of the species studied by this author were American varieties.
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