Bioorthogonal ligation with functionalized fluorescent dyes enables visualization of nuclei acids, proteins and metabolites in biological systems. Bright and modular azide- and alkyne-functionalized dyes are therefore needed to expand the fluorescence imaging capabilities of bioorthogonal ligation methods. We describe the concise synthesis of clickable fluorescent dyes based on 2-dicyanomethylene-3-cyano-2,5-dihydrofuran fluorophores and demonstrate their utility for multicolor imaging of azide- and alkyne-modified proteins as well as FRET studies. © 2009 The Royal Society of Chemistry.
CITATION STYLE
Tsou, L. K., Zhang, M. M., & Hang, H. C. (2009). Clickable fluorescent dyes for multimodal bioorthogonal imaging. Organic and Biomolecular Chemistry, 7(24), 5055–5058. https://doi.org/10.1039/b917119n
Mendeley helps you to discover research relevant for your work.