Cloning of cytochrome P450, CYP6P5, and CYP6AA2 from Anopheles minimus resistant to deltamethrin.

  • Rongnoparut P
  • Boonsuepsakul S
  • Chareonviriyaphap T
 et al. 
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Abstract

Two new genes in the cytochrome P450 (CYP6) family 6 with complete coding sequences were cloned and sequenced from deltamethrin-resistant Anopheles minimus, a major malaria vector in Thailand. CYP6P5 encodes a protein of 508 amino acids, while CYP6AA2 contains 505 residues. Each encoded protein contains a hydrophobic N-terminal region and a highly conserved heme-binding region typical of P450s. Alignments of deduced amino acid sequences with other insect P450 genes indicate a high degree of identity to insect CYP6 genes. Comparative mRNA expression studies using semi-quantitative RT-PCR analysis indicated that the relative amount of CYP6AA2 transcript was greater in the deltamethrin-resistant An. minimus compared to the susceptible strain. The expression of CYP6AA2 in deltamethrin-resistant mosquitoes is associated with development of deltamethrin resistance in An. minimus mosquito. The CYP6P5 transcript is equally expressed in both resistant and susceptible mosquitoes.

Author-supplied keywords

  • Amino Acid Sequence
  • Animals
  • Anopheles
  • Anopheles: genetics
  • Anopheles: physiology
  • Cloning
  • Cytochrome P-450 Enzyme System
  • Cytochrome P-450 Enzyme System: genetics
  • Drug Resistance
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Insecticides
  • Insecticides: pharmacology
  • Molecular
  • Molecular Sequence Data
  • Nitriles
  • Pyrethrins
  • Pyrethrins: pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction

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Authors

  • Pornpimol Rongnoparut

  • Soamrutai Boonsuepsakul

  • Theeraphap Chareonviriyaphap

  • Naowarat Thanomsing

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