A colorimetric method for determining low concentrations of mercaptans

  • Ellman G
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1. 1. A method for the determination of mercaptans is described. It depends on the interchange of bis(p-nitrophenyl)disulfide and mercaptan anions at pH 8.0. 2. 2. A study of the specificity of the reaction indicates that it occurs only with the S-group (actual or potential, as in thiourea). 3. 3. Heavy-metal ions interfere with the reaction by irreversibly bonding any S-ions. 4. 4. The kinetics of the reaction with cysteine and β-mercaptoethylamine were studied. 5. 5. Several applications of this reaction were made: (a) the determination of mercaptan in synthetic compounds; (b) mercaptan reactivity in proteins; and (c) determination of blood and serum concentrations. © 1958.

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  • George L. Ellman

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