Commonly Used Reagents

  • Solutions S
  • 103

    Readers

    Mendeley users who have this article in their library.
  • 0

    Citations

    Citations of this article.

Abstract

This collection describes the preparation of buffers and reagents used in the manipulation of proteins. Buffers and Stock Solutions * Ammonium acetate, 10 M * Dithiothreitol (DTT), 1 M * EDTA (ethylenediamine tetraacetic acid), 0.5 M (pH 8.0) * Ethidium bromide, 10 mg/ml * HCl, 1 M * KCl, 1 M * MgCl2, 1 M * MgSO4, 1 M * NaCl, 5 M * NaOH, 10 M * Phenol, buffered * Phosphate-buffered saline (PBS) * Potassium acetate buffer, 0.1 M * Potassium phosphate buffer, 0.1 M * SDS, 20% * Sodium acetate, 3 M * Sodium acetate buffer, 0.1 M * Sodium phosphate buffer, 0.1 M * SSC (sodium chloride/sodium citrate), 20× * TAE (Tris/acetate/EDTA) electrophoresis buffer * TBE (Tris/borate/EDTA) electrophoresis buffer * TE buffer * Tris⋅Cl [tris(hydroxymethyl)aminomethane], 1 M

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Stock Solutions

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free