Comparison of rumen fluid inoculum vs. faecal inoculum on predicted methane production using a fully automated in vitro gas production system

  • Ramin M
  • Lerose D
  • Tagliapietra F
 et al. 
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Abstract

The aim of the present study was to compare in vitro methane (CH4) production and other fermentation parameters from different substrates incubated either in rumen or faecal inoculum. Five different substrates were incubated in two different inocula and gas recordings were made using an automated in vitro gas production system. The substrates were: particulate matter obtained from reticulo-rumen (RR) digesta and faeces (FC) by wet sieving, timothy hay (H), first cut grass silage (S) and a mixture of grass silage and barley (50:50; SB). One gram of each substrate was incubated either in 60ml buffered rumen or faecal inoculum taken from Swedish lactating dairy cows for 48h. The results indicated that in vitro total gas production, predicted in vivo CH4production and the ratio of CH4production to total gas production were greater (P

Author-supplied keywords

  • Faeces
  • Hindgut fermentation
  • Methane
  • Ruminants
  • VFA

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