Complete inventory of soybean NAC transcription factors: Sequence conservation and expression analysis uncover their distinct roles in stress response

  • Pinheiro G
  • Marques C
  • Costa M
 et al. 
  • 89


    Mendeley users who have this article in their library.
  • 89


    Citations of this article.


We performed an inventory of soybean NAC transcription factors, in which 101 NAC domain-containing proteins were annotated into 15 different subgroups, showing a clear relationship between structure and function. The six previously described GmNAC proteins (GmNAC1 to GmNAC6) were located in the nucleus and a transactivation assay in yeast confirmed that GmNAC2, GmNAC3, GmNAC4 and GmNAC5 function as transactivators. We also analyzed the expression of the six NAC genes in response to a variety of stress conditions. GmNAC2, GmNAC3 and GmNAC4 were strongly induced by osmotic stress. GmNAC3 and GmNAC4 were also induced by ABA, JA and salinity but differed in their response to cold. Consistent with an involvement in cell death programs, the transient expression of GmNAC1, GmNAC5 and GmNAC6 in tobacco leaves resulted in cell death and enhanced expression of senescence markers. Our results indicate that the described soybean NACs are functionally non-redundant transcription factors involved in response to abiotic stresses and in cell death events in soybean. © 2009 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Genome
  • Glycine max
  • NAC genes
  • Plant-specific transfactors
  • Soybean
  • Stress expression pattern

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Guilherme L. Pinheiro

  • Carolina S. Marques

  • Maximiller D B L Costa

  • Pedro A B Reis

  • Murilo S. Alves

  • Claudine M. Carvalho

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free