A complete protein pattern of cellulase and hemicellulase genes in the filamentous fungus Trichoderma reesei

  • Ouyang J
  • Yan M
  • Kong D
 et al. 
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Abstract

The complete protein pattern of cellulase and hemicellulase genes was studied through the Genome-wide analysis in Trichoderma reesei. The genome database revealed the presence of 39 ORFs encoding related proteins, including 32 enzymes with a catalysis domain related to cellulases and hemicellulases and 7 related proteins with a cellulose-binding module (CBM). Ten of these encoded yet undescribed enzymes, including six novel beta-glucosidases or xylosidases, two putative xylanases and two undescribed mannases. To better illustrate the relation of these 39 related proteins, four groups were created and analyzed by phylogenetic analysis: group A corresponding to xylanases, group B belonging to mannases and acting to degrade mannan; group C containing all known and putative cellulose-degrading proteins that have highly conserved CBMs; and group D containing beta-glucosidase and beta-xylosidase. Group D was the largest group, in which 8 beta-glucosidases appeared to be non-secreted proteins.

Author-supplied keywords

  • Cellulase
  • Genes
  • Hemicellulase
  • Protein pattern
  • Trichoderma reesei

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Authors

  • Jia Ouyang

  • Ming Yan

  • Dechong Kong

  • Lin Xu

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