Computational Framework for Simulating Fluorescence Microscope Images With Cell Populations

  • Lehmussola A
  • Ruusuvuori P
  • Selinummi J
 et al. 
  • 2

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

Fluorescence microscopy combined with digital imaging constructs a basic platform for numerous biomedical studies in the field of cellular imaging. As the studies relying on analysis of digital images have become popular, the validation of image processing methods used in automated image cytometry has become an important topic. Especially, the need for efficient validation has arisen from emerging high-throughput microscopy systems where manual validation is impractical. We present a simulation platform for generating synthetic images of fluorescence-stained cell populations with realistic properties. Moreover, we show that the synthetic images enable the validation of analysis methods for automated image cytometry and comparison of their performance. Finally, we suggest additional usage scenarios for the simulator. The presented simulation framework, with several user-controllable parameters, forms a versatile tool for many kinds of validation tasks, and is freely available at http://www.cs.tut.fi/sgn/csb/simcep.

Author-supplied keywords

  • Algorithms
  • Cells, Cultured
  • Computer Simulation
  • Image Enhancement
  • Image Interpretation, Computer-Assisted
  • Microscopy, Fluorescence
  • Models, Biological
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Software
  • Software Validation
  • automated image cytometry
  • biological techniques
  • biomedical optical imaging
  • cellular biophysics
  • cellular imaging
  • digital image analysis
  • fluorescence
  • fluorescence microscopy
  • fluorescence-stained cell population
  • high-throughput microscopy system
  • image processing
  • medical image processing
  • optical microscopy

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • A Lehmussola

  • P Ruusuvuori

  • J Selinummi

  • H Huttunen

  • O Yli-Harja

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free