Confocal scanning laser microscopy measurements of atherosclerotic lesions in mice aorta. A fast evaluation method for volume determinations.

  • Andreasson A
  • Abt M
  • Jönsson-Rylander A
  • 3


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


Atherosclerotic lesion size in mice is routinely evaluated by morphometrical measurements on serial sections in order to obtain volume measurements. The technique of confocal scanning laser microscopy (CSLM) makes it possible to optically scan and thereby evaluate a tissue sample. We here describe a method for measuring lesion volume in ApoE/LDLr deficient mice at 20 and 30 weeks of age using the non-destructive procedure of CSLM. Whole mount preparations of opened aorta with the lumen side facing the cover slip were analysed under 10x magnification in a CSLM (Leica). The autofluorescence of the elastic fibres of the lamina interna as opposed to the non-fluorescing lesion was used to define the bottom and top of the lesion during scanning. Ten to forty images were collected 2.4 microm apart, depending on the size of the lesion, and the stack of images was then analysed using Imaris (Bitplane). After the CSLM evaluation, the aortas were de-mounted, embedded in paraffin, sectioned, stained in hematoxylin and eosin and the volume re-evaluated with conventional morphometry. Statistical evaluation showed that the results obtained with CSLM and the results of morphometry were positively correlated. Area measurements of the plaques using en face preparations of aorta showed that the plaque area was generally larger at the left side and a significant increase of plaque area along the length of the thoracic aorta. Our results showed that atherosclerotic lesions in mice can be quantitatively evaluated by CSLM.

Author-supplied keywords

  • Animals
  • Aorta, Thoracic
  • Aorta, Thoracic: pathology
  • Aortic Diseases
  • Aortic Diseases: pathology
  • Apolipoproteins E
  • Apolipoproteins E: genetics
  • Arteriosclerosis
  • Arteriosclerosis: pathology
  • Disease Models, Animal
  • Male
  • Mice
  • Mice, Knockout
  • Microscopy, Confocal
  • Microscopy, Confocal: methods
  • Receptors, LDL
  • Receptors, LDL: genetics

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Anne-Christine Andreasson

  • Markus Abt

  • Ann-Cathrine Jönsson-Rylander

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free