Conjugation of Methoxypolyethylene Glycol to the Surface of Bovine Red Blood Cells

  • Krajewska B
  • Sathishkumar M
  • Jeong E
 et al. 
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Abstract

The enzyme organophosphorus hydrolase (OPH) was spontaneously entrapped in carboxylethyl- or aminopropyl-functionalized mesoporous silica with rigid, uniform open-pore geometry (30 nm). This approach yielded larger amounts of protein loading and much higher specific activity of the enzyme when compared to the unfunctionalized mesoporous silica and normal porous silica with the same pore size. When OPH was incubated with the functionalized mesoporous silica, protein molecules were sequestered in or excluded from the porous material, depending on electrostatic interaction with the charged functional groups. OPH entrapped in the organically functionalized nanopores showed an exceptional high immobilization efficiency of more than 200% and enhanced stability far exceeding that of the free enzyme in solution. The combination of high protein loading, high immobilization efficiency and stability is attributed to the large and uniform pore structure, and to the optimum environment introduced by the functional groups.

Author-supplied keywords

  • *Aphthovirus/pathogenicity
  • *Hot Temperature
  • - d -glucopyranoside
  • -glucosidase
  • 4 nanoparticle
  • ??-Galactosidase
  • ??-chymotrypsin
  • ??-galactosidase
  • ??-galactosidase deactivation
  • Activation energy
  • Adsorption
  • Agarose
  • Animals
  • Applications
  • Aspergillus oryzae
  • Batch processing
  • Beads
  • Beta-Galactosidase
  • Bicinchoninic acid
  • Biocatalysis
  • Biopolymers
  • Bioreactors
  • Biotechnology
  • Biotransformations
  • Buffers
  • CDAP
  • Carbohydrates
  • Carrageenan
  • Cationic polyelectrolytes
  • Chemistry
  • Chitin
  • Chitosan
  • Chitosan beads
  • Chromatography
  • Chymotrypsin
  • Chymotrypsin: analysis
  • Chymotrypsin: metabolism
  • Collision theory
  • Colloidal stability
  • Controlled drug delivery
  • Covalent immobilization
  • Cross-linking
  • Culture Techniques
  • Diclofenac sodium
  • Double emulsion technique
  • Duolite A568
  • Encapsulation
  • Enzymatic synthesis
  • Enzyme Assays
  • Enzyme Stability
  • Enzyme catalysis
  • Enzyme immobilization
  • Enzyme reactor
  • Enzyme stabilization
  • Enzymes
  • Ferrocene
  • Fibers
  • Food emulsions
  • Galacto- oligosaccharides
  • Galactose
  • Genetic
  • Glucose biosensor
  • Glucose oxidase
  • Glutaral
  • Glutaral: pharmacology
  • Glutaraldehyde
  • Glycoside
  • Graphite
  • HLB
  • High Pressure Liquid
  • Humans
  • Hydrogel
  • Hydrogels
  • Hydrogen-Ion Concentration
  • Hydrogen-Ion Concentration: drug effects
  • Hydrolysis
  • Hydrophilization
  • Immobilisation
  • Immobilization
  • Immobilized
  • Immobilized biocatalyst
  • Immobilized enzyme
  • Immobilized enzymes
  • Immobilized: chemistry
  • Immobilized: metabolism
  • Invertase
  • Iron
  • Kinetic model
  • Kinetic parameters
  • Kinetics
  • Kluyvermyces fragilis
  • Kluyveromyces
  • Kluyveromyces fragilis
  • Kluyveromyces lactis
  • Kluyveromyces: enzymology
  • Lactase
  • Lactose
  • Lactose hydrolysis
  • Lactose intolerance
  • Lactose: chemistry
  • Lactose: metabolism
  • Lactozym
  • Lactulose
  • Magnetic beads
  • Magnetic support
  • Magnetics
  • Magnetics: methods
  • Manganese
  • Mice
  • Microspheres
  • Multiple emulsions
  • Mutation
  • Nano-environment
  • Nanoparticle
  • Nanoparticles
  • Nanoparticles: chemistry
  • Nanoparticles: ultrastructure
  • Nanotechnology
  • Nickel
  • Non-ionic surfactant Triton X-100 (??-galactosidas
  • Nonaqueous biocatalysis
  • Organic co-solvents
  • Organic solvent
  • Organic solvents
  • Organically modified sol-gel/chitosan composite ma
  • Oxides
  • PLGA
  • Physical
  • Physicochemical Phenomena
  • Polysiloxane
  • Polyvinyl Alcohol
  • Polyvinyl Alcohol: chemistry
  • Polyvinyl alcohol
  • Protein Denaturation
  • Protein Engineering
  • RNA
  • Repeated-batch
  • Resin Duolite A568
  • Review
  • Selection
  • Sephadex
  • Silanes
  • Silica
  • Silicon
  • Silicon Dioxide
  • Silicon Dioxide: chemistry
  • Sol-gel conditions
  • Sol-gel silica glass
  • Solubility
  • Solvent stability
  • Supported catalysts
  • Surface Properties
  • Swine
  • Tablets
  • Temperature
  • Time Factors
  • Titanium
  • Transdermal devices
  • Viral
  • Viral Proteins
  • Virulence
  • Virus Cultivation
  • Viscosity
  • Whey
  • Whole milk
  • Whole-cell catalysis
  • Zinc
  • acrylic acid
  • alginate
  • b -galactosidase á galacto-oligosaccharide
  • b-galactosidase
  • beta-Galactosidase
  • beta-Galactosidase: chemistry
  • beta-Galactosidase: metabolism
  • bicontinuous microemulsion
  • biomedical applications
  • bioreactors
  • biosensor
  • bovine red blood cells
  • carbodiimide coupling
  • cellobiase
  • characterization
  • chitin
  • chitosan
  • coatings
  • complexation
  • condensation
  • crystallinity
  • deacetylation degree
  • deactivation
  • diazotization
  • dispersion
  • emulsion
  • enzymatic hydrolysis
  • enzymatic synthesis
  • enzyme electrode
  • enzyme immobilization
  • ethylene glycol
  • fl-galactosidase
  • free radical polymerization
  • functional characterization
  • functional groups
  • galacto-oligosaccharides
  • gelation
  • glycidyl methacrylate
  • graft membranes
  • grafting
  • hydrogels
  • immobilization
  • immobilized á lactose á
  • lactose hydrolysis
  • macromonomer
  • magnetic fe 3 o
  • mass balance
  • methacrylic acid
  • methoxypoly
  • methyl methacrylate
  • micro-
  • microspheres
  • molecular weight
  • n -hexyl-
  • n -vinylisobutyramide
  • nano-calcium carbonate
  • nanosphere
  • packed-bed
  • packed-bed immobilized enzyme reactor
  • penicillium canescens
  • phase sepa-
  • piezoelectric crystal
  • pmma-f
  • poly
  • polyelectrolytes
  • polygma
  • porous
  • product inhibition
  • protease
  • purification
  • review
  • seed
  • stealth ery-
  • structure
  • suspension
  • thermosensitive property
  • thioglycolic acid
  • throcytes
  • transfusion
  • transgalactosylation
  • triethylene glycol dimethacrylate
  • waveguide
  • á
  • α-amylase
  • β - d -galactosidase
  • β -galactosidase
  • β-Galactosidase
  • β-galactosidase

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Authors

  • Barbara Krajewska

  • M. Sathishkumar

  • E.S. Jeong

  • S.E. Yun

  • S.P. Mun

  • J.F. Rusling

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