Conserved and specific responses to hypoxia in Aspergillus oryzae and Aspergillus nidulans determined by comparative transcriptomics

  • Terabayashi Y
  • Shimizu M
  • Kitazume T
 et al. 
  • 27


    Mendeley users who have this article in their library.
  • 13


    Citations of this article.


Hypoxia imposes stress on filamentous fungi that require oxygen to proliferate. Global transcription analysis of Aspergillus oryzae grown under hypoxic conditions found that the expression of about 50% of 4,244 affected genes was either induced or repressed more than 2-fold. A comparison of these genes with the hypoxically regulated genes of Aspergillus nidulans based on their predicted amino acid sequences classified them as bi-directional best hit (BBH), one-way best hit (extra homolog, EH), and no-hit (non-syntenic genes, NSG) genes. Clustering analysis of the BBH genes indicated that A. oryzae and A. nidulans down-regulated global translation and transcription under hypoxic conditions, respectively. Under hypoxic conditions, both fungi up-regulated genes for alcohol fermentation and the γ-aminobutyrate shunt of the tricarboxylate cycle, whereas A. oryzae up-regulated the glyoxylate pathway, indicating that both fungi eliminate NADH accumulation under hypoxic conditions. The A. oryzae NS genes included specific genes for secondary and nitric oxide metabolism under hypoxic conditions. This comparative transcriptomic analysis discovered common and strain-specific responses to hypoxia in hypoxic Aspergillus species.

Author-supplied keywords

  • DNA microarray
  • Denitrification
  • Environmental stress
  • Fermentation
  • Gluconeogenesis
  • Glycolysis

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Yasunobu Terabayashi

  • Motoyuki Shimizu

  • Tatsuya Kitazume

  • Shunsuke Masuo

  • Tatsuya Fujii

  • Naoki Takaya

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free