Contribution of extracranial lymphatics and arachnoid villi to the clearance of a CSF tracer in the rat.

  • Boulton M
  • Flessner M
  • Armstrong D
 et al. 
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The objective of this study was to determine the relative roles of arachnoid villi and cervical lymphatics in the clearance of a cerebrospinal fluid (CSF) tracer in rats. 125I-labeled human serum albumin (125I-HSA; 100 micrograms) was injected into one lateral ventricle, and an Evans blue dye-rat protein complex was injected intravenously. Arterial blood was sampled for 3 h. Immediately after this, multiple cervical vessels were ligated in the same animals, and plasma recoveries were monitored for a further 3 h after the intracerebroventricular injection of 100 micrograms 131I-HSA. Tracer recovery in plasma at 3 h averaged (%injected dose) 0.697 +/- 0.042 before lymphatic ligation and dropped significantly to 0.357 +/- 0. 060 after ligation. Estimates of the rate constant associated with the transport of the CSF tracer to plasma were also significantly lower after obstruction of cervical lymphatics (from 0.584 +/- 0. 072/h to 0.217 +/- 0.056/h). No significant changes were observed in sham-operated animals. Assuming that the movement of the CSF tracer to plasma in lymph-ligated animals was a result of arachnoid villi clearance, we conclude that arachnoid villi and extracranial lymphatic pathways contributed equally to the clearance of the CSF tracer from the cranial vault.

Author-supplied keywords

  • Animals
  • Arachnoid
  • Arachnoid: physiology
  • Biological Transport
  • Biological Transport: physiology
  • Injections, Intraventricular
  • Iodine Radioisotopes
  • Ligation
  • Lymphatic System
  • Lymphatic System: physiology
  • Male
  • Neck
  • Rats
  • Rats, Wistar
  • Serum Albumin
  • Serum Albumin: analysis
  • Serum Albumin: cerebrospinal fluid
  • Serum Albumin: pharmacokinetics

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  • M Boulton

  • M Flessner

  • D Armstrong

  • R Mohamed

  • J Hay

  • M Johnston

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