Control of focal adhesion dynamics by material surface characteristics

  • Diener A
  • Nebe B
  • Luthen F
 et al. 
  • 6

    Readers

    Mendeley users who have this article in their library.
  • N/A

    Citations

    Citations of this article.

Abstract

The mechanisms of cell adhesion to the extracellular matrix (ECM) which are of fundamental importance for function, survival, and growth of cells involve the formation of focal adhesions to facilitate integrin signaling. Recently, it became evident that focal adhesions are not stable but move to enable cell migration and ECM formation. We examined the number, size, and dynamic behavior of focal adhesions in living MG-63 osteoblastic cells, which were cultured on titanium surfaces with different roughnesses and on stainless steel (SS). As a marker for focal adhesions we used GFP-tagged vinculin, a cytoskeletal protein. Focal adhesions were smaller on titanium and on SS than on collagen-coated glass coverslips. The corundum-blasted rough surface of titanium induced the smallest adhesions. On all the surfaces that we have tested, we observed a mobility of focal adhesions. On collagen-coated coverslips focal adhesions moved with a speed of 60 nm/min. The speed was reduced on titanium and still more restricted on SS. The topography did not affect the mobility of focal adhesions. We conclude that on the material surfaces that we have studied a reduced mobility of focal adhesions may strengthen the linkages between cell and ECM but impair the ability to dynamically organize and remodel the EM The results may have a great impact in the functional evaluation of tailored biomaterial surfaces for the application in tissue engineering. (C) 2004 Elsevier Ltd. All rights reserved.

Author-supplied keywords

  • BIOMATERIALS
  • CELL-MATRIX ADHESIONS
  • CROSSTALK
  • FIBROBLASTS
  • FIBRONECTIN
  • INTEGRINS
  • MIGRATION
  • MORPHOLOGY
  • RGD
  • TOPOGRAPHY
  • focal adhesion
  • mobility
  • osteoblast
  • surface topography
  • titanium
  • vinculin

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • A Diener

  • B Nebe

  • F Luthen

  • P Becker

  • U Beck

  • H G Neumann

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free