Coomassie brilliant blue staining of lipids on thin-layer plates

  • Nakamura K
  • Handa S
  • 23

    Readers

    Mendeley users who have this article in their library.
  • 172

    Citations

    Citations of this article.

Abstract

Coomassie brilliant blue staining of lipids on silica gel thin-layer chromatography plates is described. This stain proved to be useful for the wide-range detection of simple and complex lipids on thin-layer plates. It can stain several classes of lipids, including cholesterol, cholesterol esters, glycerides, phospholipids, ceramides, and neutral and acidic glycosphingolipids. It stains the spots evenly without a corrosive reagent, and is simple to use and suitable for storage. The visual detection limits of this stain for lipids were 0.05 to 0.5 μg. © 1984.

Author-supplied keywords

  • gangliosides
  • glycolipids
  • lipids
  • phospholipids
  • stain
  • thin-layer chromatography

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Kazuo Nakamura

  • Shizuo Handa

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free