Culturing thick brain slices: An interstitial 3D microperfusion system for enhanced viability

  • Rambani K
  • Vukasinovic J
  • Glezer A
 et al. 
  • 155

    Readers

    Mendeley users who have this article in their library.
  • 36

    Citations

    Citations of this article.

Abstract

Brain slice preparations are well-established models for a wide spectrum of in vitro investigations in the neuroscience discipline. However, these investigations are limited to acute preparations or thin organotypic culture preparations due to the lack of a successful method that allows culturing of thick organotypic brain slices. Thick brain slice cultures suffer necrosis due to ischemia deep in the tissue resulting from a destroyed circulatory system and subsequent diffusion-limited supply of nutrients and oxygen. Although thin organotypic brain slice cultures can be successfully cultured using a well-established roller-tube method (a monolayer organotypic culture) (Gahwiler B H. Organotypic monolayer cultures of nervous tissue. J Neurosci Methods. 1981; 4: 329-342) or a membrane-insert method (up to 1-4 cell layers,

Author-supplied keywords

  • Convection enhanced perfusion
  • Ischemia
  • Microfluidic
  • Nervous tissue cultures
  • Organotypic slice cultures
  • Perfusion
  • Thick brain slice cultures

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text

Authors

  • Komal Rambani

  • Jelena Vukasinovic

  • Ari Glezer

  • Steve M. Potter

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free