Culturing thick brain slices: An interstitial 3D microperfusion system for enhanced viability

  • Rambani K
  • Vukasinovic J
  • Glezer A
 et al. 
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Abstract

Brain slice preparations are well-established models for a wide spectrum of in vitro investigations in the neuroscience discipline. However, these investigations are limited to acute preparations or thin organotypic culture preparations due to the lack of a successful method that allows culturing of thick organotypic brain slices. Thick brain slice cultures suffer necrosis due to ischemia deep in the tissue resulting from a destroyed circulatory system and subsequent diffusion-limited supply of nutrients and oxygen. Although thin organotypic brain slice cultures can be successfully cultured using a well-established roller-tube method (a monolayer organotypic culture) (Gahwiler B H. Organotypic monolayer cultures of nervous tissue. J Neurosci Methods. 1981; 4: 329-342) or a membrane-insert method (up to 1-4 cell layers,

Author-supplied keywords

  • Convection enhanced perfusion
  • Ischemia
  • Microfluidic
  • Nervous tissue cultures
  • Organotypic slice cultures
  • Perfusion
  • Thick brain slice cultures

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Authors

  • Komal Rambani

  • Jelena Vukasinovic

  • Ari Glezer

  • Steve M. Potter

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