Deglycosylation of glucose oxidase to improve biosensors and biofuel cells

  • Prévoteau A
  • Courjean O
  • Mano N
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Abstract

We demonstrate that a more efficient redox hydrogel structure can be achieved by engineering the size and the surface charge of the bioelectrocatalyst. Deglycosylated glucose oxidase (GOx) modified electrode exhibits higher current density than native GOx, for the same molar composition of the hydrogel. This improvement is very likely due to a more efficient hydrogel structure rather than a better intrinsic electron transfer between the FAD/FADH2 redox center and the redox mediator. © 2009 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Biofuel cells
  • Biosensors
  • Deglycosylation
  • Enzyme engineering
  • Glucose oxidase

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