Diffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution. After dehydration, there is a spectacular improvement, with the diffraction pattern extending to 2 Å resolution. This and other recent results show that dehydration is a simple, rapid, and inexpensive approach to convert poor quality crystals into diffraction quality crystals.
CITATION STYLE
Heras, B., Edeling, M. A., Byriel, K. A., Jones, A., Raina, S., & Martin, J. L. (2003). Dehydration converts DsbG crystal diffraction from low to high resolution. Structure, 11(2), 139–145. https://doi.org/10.1016/S0969-2126(03)00005-4
Mendeley helps you to discover research relevant for your work.