Detection of alternative lengthening of telomeres by telomere quantitative PCR

  • Lau L
  • Dagg R
  • Henson J
 et al. 
  • 63


    Mendeley users who have this article in their library.
  • 27


    Citations of this article.


Alternative lengthening of telomeres (ALT) is one of the two known telomere length maintenance mech- anisms that are essential for the unlimited prolifer- ation potential of cancer cells. Existing methods for detecting ALT in tumors require substantial amounts of tumor material and are labor intensive, making it difficult to study prevalence and prognos- tic significance of ALT in large tumor cohorts. Here, we present a novel strategy utilizing telomere quan- titative PCR to diagnose ALT. The protocol is more rapid than conventional methods and scrutinizes two distinct characteristics of ALT cells concur- rently: long telomeres and the presence of C-circles (partially double-stranded circles of telo- meric C-strand DNA). Requiring only 30ng of genomic DNA, this protocol will facilitate large- scale studies of ALT in tumors and can be readily adopted by clinical laboratories.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Jeremy HensonUniversity of New South Wales

  • Loretta M.S. Lau

  • Rebecca A. Dagg

  • Amy Y.M. Au

  • Janice A. Royds

  • Roger R. Reddel

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free