Detection of Francisella tularensis in blood by polymerase chain reaction

  • Long G
  • Oprandy J
  • Narayanan R
 et al. 
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Abstract

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/microliter of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

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