Detection of low levels of human immunodeficiency virus (HIV) may be critical for early diagnosis of pediatric HIV infection by use of dried blood spots

  • Walter J
  • Kuhn L
  • Semrau K
 et al. 
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We compared a DNA-based assay with a total nucleic acid-based assay for early detection of infant human immunodeficiency virus (HIV) infection. The codetection of DNA and RNA did not result in an overall higher sensitivity compared to that of DNA alone. Discordant results were associated with low levels of HIV DNA, indicating that the sample amount may be critical.

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  • Jan Walter

  • Louise Kuhn

  • Katherine Semrau

  • Don W. Decker

  • Moses Sinkala

  • Chipepo Kankasa

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