Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR

Abstract

Key words ABSTRACT: S. typhimurium-S. enteritidis-S. Kentucky-chicken meat-Multiplex-PCR The present study was conducted for identification of molecular characterization of Salmonella enterica isolated from chicken meat by using multiplex-PCR isolated from 200 samples of fresh chicken meat and 100 samples of ready to eat chicken meat. Samples were assessed by performing the pre-enrichment and enrichment culture, biochemically characterized by the analytical profiling index (API 20E system). The primers were selected for invA, stn, avrA, sefA, fliC and stm 4495 genes, specific for the detection of Salmonella enteritidis, Salmonella typhimurium and Salmonella kentucky. Standard bacteriological examination revealed that 7 S. enterica isolated from 200 chicken meats with a percentage of (3.5%), including 5 S. typhimurium with a percentage of (2.5%) and 1 for each of S. enteritidis and S. kentucky with a percentage of (0.5 %), while could not isolate S. enterica from ready to eat chicken meat. The results of multiplex PCR for the three isolated serotypes of Salmonella enterica (1 for each S. typhimurium, S. Kentucky and S. enteritidis) could detect the universal gene (invA) and virulence genes (avrA and stn) in all examined serotypes, while (fliC) gene was detected in both S. typhimurium and S. kentucky only while not detected in S. enteritidis, (stm 4495) gene was detected only in S. typhimurium but was not detected in S. kentucky and S. enteritidis. (sefA) gene was detected only in S. enteritidis but was not detected in S. typhimurium and S. kentucky.