Detection and structural characterization of natural Ara h 7, the third peanut allergen of the 2S albumin family

  • Schmidt H
  • Krause S
  • Gelhaus C
 et al. 
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Abstract

In recent years, several novel relevant peanut allergens have been identified. Among those, a new member of the conglutin family was cloned by a phage display approach and initially annotated as Ara h 7.0101. Later, however, recloning of Ara h 7 revealed an alternate isoform, termed Ara h 7.0201. Because the natural Ara h 7 counterpart had not been found at the protein level in peanut extracts, the aim of the present study was to search for authentic natural Ara h 7 protein(s). To this end, enriched low molecular mass proteins (

Author-supplied keywords

  • 2D electrophoresis
  • Ara h 7
  • Arachis hypogaea
  • N-terminal sequencing
  • Peanut allergens
  • mass spectrometry
  • phage display

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Authors

  • Hendrik Schmidt

  • Susanne Krause

  • Christoph Gelhaus

  • Arnd Petersen

  • Ottmar Janssen

  • Wolf Meinhard Becker

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