Detection of transovarial dengue virus from field-caught Aedes aegypti and Ae. albopictus larvae using C6/36 cell culture and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques

  • Rohani A
  • Zamree I
  • Lee H
 et al. 
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Abstract

Larvae of Aedes aegypti and Aedes albopictus were collected from a wide variety of artificial containers. Most samples were collected from used tyres and water-holding containers located in residential urban or rural areas. The identified mosquito larvae were pooled according to the species, date and locality and stored at -70 (degrees)C. A total of 378 of pools of Ae. aegypti and 553 pools of Ae. albopictus were collected. Virus isolation was carried out using cell culture (C6/36 done) of Ae. albopictus and virus detection by reverse-transcriptase polymerase chain reaction (RT-PCR). Transovarial transmission of dengue virus was demonstrated in both Ae. aegypti and Ae. albopictus in nature. Infected larvae were recovered from 16 localities (10 in Terengganu; 5 in Kuala Lumpur and 1 in Pahang). The study showed that both the cell culture and RT-PCR techniques can be used to detect dengue virus from mosquito larvae.

Author-supplied keywords

  • Aedes aegypti
  • Aedes albopictus
  • Dengue
  • Malaysia
  • Transovarial transmission

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  • PUI: 352685272
  • ISSN: 1020895X
  • SCOPUS: 2-s2.0-56249098348
  • SGR: 56249098348
  • ISBN: 1020-895X

Authors

  • A. Rohani

  • I. Zamree

  • H. L. Lee

  • I. Mustafakamal

  • M. J. Norjaiza

  • D. Kamilan

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