Determination of carbohydrates by condensation with 3-methyl-2-benzothiazolinonehydrazone

  • Honda S
  • Nishimura Y
  • Chiba H
 et al. 
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Abstract

The condensation reactions obtained by heating carbohydrates with 3-methyl-2-benzothiazolinonehydrazone (MBTH) in alkaline solution containing 2-methoxyethanol are utilized. The absorbance of the product is measured at 390 nm against the reagent blank. D-Glucose (50-350 nmol) can be determined accurately with coefficients of variation less than 1.5%. Other reducing carbohydrates give similar results. The selectivity of this method is discussed. © 1981.

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Authors

  • Susumu Honda

  • Yoshiko Nishimura

  • Hirokazu Chiba

  • Kazuaki Kakehi

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