Determination of carbohydrates by condensation with 3-methyl-2-benzothiazolinonehydrazone

Abstract

The condensation reactions obtained by heating carbohydrates with 3-methyl-2-benzothiazolinonehydrazone (MBTH) in alkaline solution containing 2-methoxyethanol are utilized. The absorbance of the product is measured at 390 nm against the reagent blank. D-Glucose (50-350 nmol) can be determined accurately with coefficients of variation less than 1.5%. Other reducing carbohydrates give similar results. The selectivity of this method is discussed. © 1981.