Determination of pesticides fenoxycarb and permethrin by sequential injection chromatography using miniaturized monolithic column

  • Chocholouš P
  • Šatínský D
  • Sladkovský R
 et al. 
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Abstract

Sequential injection chromatography system equipped with miniaturized 10 mm monolithic column was used for fast simultaneous determination of two pesticides-fenoxycarb (FC) and permethrin (PM). The system was composed of a commercial sequential injection analysis (SIA) system (FIAlab® 3000, 6-port selection valve and 5.0 mL syringe pump), commercially available column Chromolith™ RP-18e (10 mm × 4.6 mm i.d.) (Merck®, Germany) and CCD UV-vis detector (USB 2000, Ocean-optics) with 1.0 cm Z-flow cell, absorbance was monitored at 225 nm. The mobile phase used for analysis was acetonitrile/water (60:40, v/v), flow rates were 0.6 mL min-1 for elution of fenoxycarb and 1.2 mL min-1 for elution of permethrin. For each analysis 4.8 mL of mobile phase was used. The chromatographic resolution between both compounds was >8 and analysis time was

Author-supplied keywords

  • Chromolith™ monolithic column
  • Fenoxycarb
  • Permethrin
  • Pesticides
  • Sequential injection chromatography (SIC)
  • Spectrophotometric determination

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