Determining paternity in polyploids: Hexaploid simulation studies

  • Buteler M
  • LaBonte D
  • Macchiavelli R
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Abstract

The breeding of new sweet potato varieties is a highly inefficient
process, confounded by incompatibility, poor fertility, open- pollination
and hexaploidy. Upwards of 12-20 lines are combined in open pollinated
nurseries based on horticulturally important characteristics. After
several years of selection most progeny can be traced back to just
3 or 4 maternal lines. A method that would identify the paternal
parent of superior progeny would enable breeders to combine parents
that exhibit superior combining ability in more efficient, smaller
nurseries. The objective of this work is to explore by means of computer
simulation the application of genealogy reconstruction techniques
on hexaploid individuals based on co-dominant marker data. The progeny
obtained from each female parent is categorically assigned to each
male with non-zero exclusion probability based on its paternity likelihood.
Computer simulations show that even with polysomic segregation types,
it is possible to discriminate between putative parents with few
errors or mis- assignments. The number of loci scored for a 10 parent
population should not be less than 20 in the case of 3 alleles per
locus, and no more than 10 loci for a five allele model. An increment
in the number of alleles or loci increases the discriminatory power
with the number of alleles yielding a far more important effect than
the number of loci. This study also demonstrates the feasibility
of using simulations to determine the minimum requirements, i.e.
number of loci to be genotyped, for unambiguous parentage allocation
in polyploids.

Author-supplied keywords

  • Codominant markers
  • Genealogy reconstruction
  • Most likely male parent
  • Polyploid
  • Polysomic segregation

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Authors

  • Mario I. Buteler

  • Don R. LaBonte

  • Raúl E. Macchiavelli

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