Development and application of a DNA microarray-based yeast two-hybrid system

  • Suter B
  • Fontaine J
  • Yildirimman R
 et al. 
  • 5


    Mendeley users who have this article in their library.
  • N/A


    Citations of this article.


The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames, proof-of-principle Y2H interaction screens were performed for the human neurodegenerative disease proteins huntingtin and ataxin-1. Using systematic controls for unspecific Y2H results and quantitative benchmarking, we identified and scored a large number of known and novel partner proteins for both huntingtin and ataxin-1. Moreover, we show that this parallelized screening procedure and the global inspection of Y2H interaction data are uniquely suited to define specific PPI patterns and their alteration by disease-causing mutations in huntingtin and ataxin-1. This approach takes advantage of the specificity and flexibility of DNA microarrays and of the existence of solid-related statistical methods for the analysis of DNA microarray data, and allows a quantitative approach toward interaction screens in human and in model organisms.

Author-supplied keywords

  • Humans
  • Mendeley Import (Oct 29)
  • Mutation
  • Nerve Tissue Proteins
  • Nerve Tissue Proteins: genetics
  • Nerve Tissue Proteins: metabolism
  • Nuclear Proteins
  • Nuclear Proteins: genetics
  • Nuclear Proteins: metabolism
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotide Array Sequence Analysis: methods
  • Open Reading Frames
  • Protein Interaction Maps
  • Two-Hybrid System Techniques
  • Yeasts
  • Yeasts: genetics
  • new stuff.ris

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Bernhard Suter

  • Jean-Fred Fontaine

  • Reha Yildirimman

  • Tamás Raskó

  • Martin H Schaefer

  • Axel Rasche

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free