Development of a cryopreservation protocol for type A spermatogonia

  • Izadyar F
  • Matthijs-Rijsenbilt J
  • Den Ouden K
 et al. 
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The aim of this study was to develop a cryopreservation protocol for type A spermatogonia. Testes from 5- to 7-month-old calves were collected, and type A spermatogonia were isolated using two-step enzymatic digestion and Percoll separation. Cells were resuspended in minimum essential medium (MEM) supplemented with 1% bovine serum albumin (BSA) in a final concentration of 6 x 10(6) per mL, and the effects of different cryoprotectants and freezing protocols were tested. Cells frozen/thawed in medium containing 10% fetal calf serum (FCS) and 1.4 M glycerol or dimethyl sulfoxide (DMSO) had a significantly (P

Author-supplied keywords

  • Freezing
  • Spermatogonial stem cells
  • Transplantation

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  • Fariborz Izadyar

  • J. J. Matthijs-Rijsenbilt

  • Krista Den Ouden

  • Laura B. Creemers

  • Henri Woelders

  • Dirk G. De Rooij

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