Abstract
The aim of this study was to develop a cryopreservation protocol for type A spermatogonia. Testes from 5- to 7-month-old calves were collected, and type A spermatogonia were isolated using two-step enzymatic digestion and Percoll separation. Cells were re-suspended in minimum essential medium (MEM) supplemented with 1% bovine serum albumin (BSA) in a final concentration of 6 × 106 per mL, and the effects of different cryoprotectants and freezing protocols were tested. Cells frozen/thawed in medium containing 10% fetal calf serum (FCS) and 1.4 M glycerol or dimethyl sulfoxide (DMSO) had a significantly (P
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Izadyar, F., Matthijs-Rijsenbilt, J. J., Den Ouden, K., Creemers, L. B., Woelders, H., & De Rooij, D. G. (2002). Development of a cryopreservation protocol for type A spermatogonia. Journal of Andrology, 23(4), 537–545. https://doi.org/10.1002/j.1939-4640.2002.tb02276.x
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