Development and Evaluation of Fluorogenic TaqMan Reverse Transcriptase PCR Assays for Detection of Dengue Virus Types 1 to 4

  • Ito M
  • Takasaki T
  • Yamada K
 et al. 
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Abstract

The fluorogenic {TaqMan} reverse transcriptase {PCR} {(RT-PCR)} assay was developed for detecting each of the dengue virus {(DV)} types 1 to 4. {DV} genome was detected in all the 35 serum samples from confirmed dengue cases by the {TaqMan} {RT-PCR,} although it was not detected in 13 and 21% by conventional type-specific and cross-reactive {RT-PCR,} respectively.

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Authors

  • Mikako Ito

  • Tomohiko Takasaki

  • Ken-ichiro Yamada

  • Reiko Nerome

  • Shigeru Tajima

  • Ichiro Kurane

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