Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4.

  • Ito M
  • Takasaki T
  • Yamada K
 et al. 
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Abstract

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

Author-supplied keywords

  • Dengue
  • Dengue Virus
  • Dengue Virus: classification
  • Dengue Virus: genetics
  • Dengue Virus: isolation & purification
  • Dengue: virology
  • Fluorescent Dyes
  • Humans
  • RNA
  • Reverse Transcriptase Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction: m
  • Sensitivity and Specificity
  • Taq Polymerase
  • Viral
  • Viral: blood

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Authors

  • Mikako Ito

  • Tomohiko Takasaki

  • Ken-ichiro Yamada

  • Reiko Nerome

  • Shigeru Tajima

  • Ichiro Kurane

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