Development of PCR assays for the detection and differentiation of Fusarium sporotrichioides and Fusarium langsethiae

  • Wilson A
  • Simpson D
  • Chandler E
 et al. 
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Abstract

Isolates of the type-A trichothecene producing Fusarium sporotrichioides and Fusarium langsethiae were grouped and differentiated in a phylogenetic tree using ITS sequence dissimilarity. An attempt was made to develop a PCR-based assay for the detection and differentiation of Fusarium sporotrichiodes from other Fusarium species using the 5′-region of the tri5 gene as a template. However, this assay was unable to differentiate, to a satisfactory level, between isolates of Fusarium sporotrichioides and Fusarium langsethiae, providing further genetic evidence for their close genetic relationship. A robust and repeatable PCR-assay was developed for the detection and differentiation of both species based on sequence determined from differentially amplified RAPD-PCR products. These assays were able to detect both species in samples of grain taken from the field. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Fusarium
  • PCR
  • Trichothecenes

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Authors

  • Anthony Wilson

  • Duncan Simpson

  • Elizabeth Chandler

  • Phil Jennings

  • Paul Nicholson

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