Differences in signaling molecule organization between naive and memory CD4+ T lymphocytes

  • Watson A
  • Lee W
  • 4

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Abstract

The immunological synapse is a highly organized complex formed at the junction between Ag-specific T cells and APCs as a prelude to cell activation. Although its exact role in modulating T cell signaling is unknown, it is commonly believed that the immunological synapse is the site of cross-talk between the T cell and APC (or target). We have examined the synapses formed by naive and memory CD4 cells during Ag-specific cognate interactions with APCs. We show that the mature immunological synapse forms more quickly during memory T cell activation. We further show that the composition of the synapse found in naive or memory cell conjugates with APCs is distinct with the tyrosine phosphatase, CD45, being a more integral component of the mature synapses formed by memory cells. Finally, we show that signaling molecules, including CD45, are preassociated in discrete, lipid-raft microdomains in resting memory cells but not in naive cells. Thus, enhanced memory cell responses may be due to intrinsic properties of signaling molecule organization.

Author-supplied keywords

  • *Immunologic Memory
  • *Signal Transduction
  • Animals
  • Antigen-Presenting Cells/physiology
  • Antigens, CD45/analysis
  • CD4-Positive T-Lymphocytes/*immunology/physiology
  • Female
  • Male
  • Membrane Microdomains/chemistry
  • Mice
  • Mice, Inbred BALB C
  • Protein-Tyrosine Kinase/physiology
  • Receptors, Antigen, T-Cell/physiology
  • Support, U.S. Gov't, P.H.S.
  • Synapses/physiology

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Authors

  • A R Watson

  • W T Lee

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