Differences in signaling molecule organization between naive and memory CD4+ T lymphocytes

  • Watson A
  • Lee W
  • 2

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Abstract

The immunological synapse is a highly organized complex formed at the junction between Ag-specific T cells and APCs as a prelude to cell activation. Although its exact role in modulating T cell signaling is unknown, it is commonly believed that the immunological synapse is the site of cross-talk between the T cell and APC (or target). We have examined the synapses formed by naive and memory CD4 cells during Ag-specific cognate interactions with APCs. We show that the mature immunological synapse forms more quickly during memory T cell activation. We further show that the composition of the synapse found in naive or memory cell conjugates with APCs is distinct with the tyrosine phosphatase, CD45, being a more integral component of the mature synapses formed by memory cells. Finally, we show that signaling molecules, including CD45, are preassociated in discrete, lipid-raft microdomains in resting memory cells but not in naive cells. Thus, enhanced memory cell responses may be due to intrinsic properties of signaling molecule organization

Author-supplied keywords

  • Animals
  • Antigen-Presenting Cells
  • Antigens
  • Antigens,CD45
  • CD4-Positive T-Lymphocytes
  • Cells
  • Female
  • Immunologic Memory
  • Lymphocytes
  • Male
  • Membrane Microdomains
  • Memory
  • Mice
  • Mice,Inbred BALB C
  • Protein-Tyrosine Kinase
  • Protein-Tyrosine Kinases
  • Receptors,Antigen,T-Cell
  • Research
  • Signal Transduction
  • Synapses
  • T-Lymphocytes
  • Tyrosine
  • ZAP-70 Protein-Tyrosine Kinase
  • analysis
  • chemistry
  • immunology
  • physiology

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  • PMID: 15210756

Authors

  • A R Watson

  • W T Lee

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