The discovery of a werner helicase interacting protein (WHIP) association with the nuclear pore complex

  • Kaur S
  • White T
  • DiGuilio A
 et al. 
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Abstract

The gateway for molecular trafficking between the cytoplasm and the nucleus is the Nuclear Pore Complex (NPC). Through mass spectral analysis of the isolated Nuclear Pore Nup107-160 subcomplex, we discovered an in vivo interaction with Werner's Helicase Interacting Protein 1, (WRNIP1 or WHIP). WHIP was originally identified as a binding partner of Werner protein (WRN), which functions to maintain genome stability and is responsible for the progeria disease, Werner syndrome. We established the reciprocal isolation of Nup107 by alpha-WHIP. WHIP was found in purified Nuclear Envelope (NE) fractions treated with DNase/RNase/Heparin. We demonstrated by immunofluorescence microscopy that WHIP is located at the nuclear rim as well as punctate regions in the nuclear matrix. Ultimately, synchronized cells show a dynamic association between WHIP and the Nup107-160 subcomplex through the cell cycle without an interaction with WRN. We thus identify WHIP as a partner/component of the NE/NPC and set forth to investigate a role for the protein positioned at the NPC.

Author-supplied keywords

  • Mitosis
  • Nuclear pore complex
  • Nucleoporins
  • Nup 107-160 subcomplex
  • WHIP
  • WRNIP
  • Werner helicase interacting protein

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Authors

  • Simarna Kaur

  • Tommy E. White

  • Amanda L. DiGuilio

  • Joseph S. Glavy

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