Distinct roles of class I and class III phosphatidylinositol 3-kinases in phagosome formation and maturation

  • Vieira O
  • Botelho R
  • Rameh L
 et al. 
  • 127


    Mendeley users who have this article in their library.
  • 341


    Citations of this article.


Phagosomes acquire their microbicidal properties by fusion with lysosomes. Products of phosphatidylinositol 3-kinase (PI 3-kinase) are required for phagosome formation, but their role in maturation is unknown. Using chimeric fluorescent proteins encoding tandem FYVE domains, we found that phosphatidylinositol 3-phosphate (PI[3]P) accumulates greatly but transiently on the phagosomal membrane. Unlike the 3'-phosphoinositides generated by class I PI 3-kinases which are evident in the nascent phagosomal cup, PI(3)P is only detectable after the phagosome has sealed. The class III PI 3-kinase VPS34 was found to be responsible for PI(3)P synthesis and essential for phagolysosome formation. In contrast, selective ablation of class I PI 3-kinase revealed that optimal phagocytosis, but not maturation, requires this type of enzyme. These results highlight the differential functional role of the two families of kinases, and raise the possibility that PI(3)P production by VPS34 may be targeted during the maturation arrest induced by some intracellular parasites.

Author-supplied keywords

  • EEA1
  • FYVE domain
  • Lysosomes
  • Phagocytosis
  • Phosphatidylinositol

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document


  • Roberto BotelhoRyerson University

  • Otilia V. Vieira

  • Lucia Rameh

  • Saskia M. Brachmann

  • Tsuyoshi Matsuo

  • Howard W. Davidson

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free