A distributed cell division counter reveals growth dynamics in the gut microbiota

  • Myhrvold C
  • Kotula J
  • Hicks W
 et al. 
  • 113


    Mendeley users who have this article in their library.
  • 8


    Citations of this article.


Microbial population growth is typically measured when cells can be directly observed, or when death is rare. However, neither of these conditions hold for the mammalian gut microbiota, and, therefore, standard approaches cannot accurately measure the growth dynamics of this community. Here we introduce a new method (distributed cell division counting, DCDC) that uses the accurate segregation at cell division of genetically encoded fluorescent particles to measure microbial growth rates. Using DCDC, we can measure the growth rate of Escherichia coli for >10 consecutive generations. We demonstrate experimentally and theoretically that DCDC is robust to error across a wide range of temperatures and conditions, including in the mammalian gut. Furthermore, our experimental observations inform a mathematical model of the population dynamics of the gut microbiota. DCDC can enable the study of microbial growth during infection, gut dysbiosis, antibiotic therapy or other situations relevant to human health.

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Cameron Myhrvold

  • Jonathan W. Kotula

  • Wade M. Hicks

  • Nicholas J. Conway

  • Pamela A. Silver

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free