DNA barcoding methods for invertebrates

  • Evans N
  • Paulay G
  • 38


    Mendeley users who have this article in their library.
  • 7


    Citations of this article.


Invertebrates comprise approximately 34 phyla, while vertebrates represent one subphylum and insects a (very large) class. Thus, the clades excepting vertebrates and insects encompass almost all of animal diversity. Consequently, the barcoding challenge in invertebrates is that of barcoding animals in general. While standard extraction, cleaning, PCR methods, and universal primers work for many taxa, taxon-specific challenges arise because of the shear genetic and biochemical diversity present across the kingdom, and because problems arising as a result of this diversity, and solutions to them, are still poorly characterized for many metazoan clades. The objective of this chapter is to emphasize general approaches, and give practical advice for overcoming the diverse challenges that may be encountered across animal taxa, but we stop short of providing an exhaustive inventory. Rather, we encourage researchers, especially those working on poorly studied taxa, to carefully consider methodological issues presented below, when standard approaches perform poorly.

Author-supplied keywords

  • CO1
  • Cytochrome c oxidase subunit I
  • DNA barcoding
  • Invertebrates

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Nathaniel Evans

  • Gustav Paulay

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free