The early embryo response to intracellular reactive oxygen species is developmentally regulated

  • Bain N
  • Madan P
  • Betts D
  • 3

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Abstract

In vitro embryo production (IVP) suffers from excessive developmental failure. Its inefficiency is linked, in part, to reactive oxygen species (ROS) brought on by high ex vivo oxygen (O-2) tensions. To further delineate the effects of ROS on IVP, the intracellular ROS levels of early bovine embryos were modulated by: (1) varying O-2 tension; (2) exogenous H2O2 treatment; and (3) antioxidant supplementation. Although O-2 tension did not significantly affect blastocyst frequencies (P>0.05), 20% O-2 accelerated the rate of first cleavage division and significantly decreased and increased the proportion of permanently arrested 2- to 4-cell embryos and apoptotic 9- to 16-cell embryos, respectively, compared with embryos cultured in 5% O-2 tension. Treatment with H2O2, when applied separately to oocytes, zygotes, 2- to 4-cell embryos or 9- to 16-cell embryos, resulted in a significant (P

Author-supplied keywords

  • antioxidant
  • apoptosis
  • bovine
  • cumulus cells
  • embryo arrest
  • hydrogen peroxide
  • hydrogen-peroxide
  • in-vitro maturation
  • induced apoptosis
  • mammalian embryos
  • oxidative stress
  • porcine oocytes
  • preimplantation bovine embryos
  • subsequent development
  • superoxide-dismutase

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Authors

  • N T Bain

  • P Madan

  • D H Betts

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