Efectos ambientales de la acuicultura intensiva y alternativas para un desarrollo sustentable

  • Buschmann A
  • Fortt A
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Abstract

The presence and diversity of IncP-1 plasmids in the influent of a Danish wastewater treatment plant was studied by PCR amplification of the trfA gene in community DNA followed by sequencing. Three sets of PCR primers were designed to amplify a 281 bp fragment of trfA from all currently sequenced IncP-1 plasmids. A neighbor-joining tree, based on a multiple alignment of 72 obtained sequences together with homologous sequences of previously published IncP-1 plasmids, revealed that all established subgroups of IncP-1 plasmids, α, β, γ and δ, were present in the wastewater treatment plant influent. Also sequences representing the recently described fifth subgroup, the ε subgroup, were detected in the wastewater. Thus, these results confirm the presence of at least five phylogenetically distinct subgroups of IncP-1 plasmids and represent the first time that sequences associated with plasmids of all of these five subgroups have been detected in a single setting. Additionally, the results confirm that wastewater constitutes a reservoir for the conjugative IncP-1 plasmids, which often harbor multiple antibiotic resistance genes. © 2009 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • Active partitioning
  • Animals
  • Anti-Bacterial Agents
  • Anti-Bacterial Agents: administration & dosage
  • Anti-Bacterial Agents: adverse effects
  • Antibiotic Prophylaxis
  • Antibiotic Prophylaxis: adverse effects
  • Antibiotic Prophylaxis: standards
  • Antibiotic Prophylaxis: utilization
  • Aquaculture
  • Aquaculture: methods
  • Aquaculture: standards
  • Bacteria
  • Bacteria: drug effects
  • Bacterial
  • Bacterial Proteins
  • Bacterial Proteins: genetics
  • Bacterial: genetics
  • Bacteroidetes
  • Bacteroidetes: genetics
  • Biotransformation
  • Chromatin immunoprecipitation
  • Conjugative transfer
  • Cooperativity
  • DNA binding
  • Drug Residues
  • Drug Residues: adverse effects
  • Drug Resistance
  • Environmental Microbiology
  • Fishes
  • Food Hypersensitivity
  • Food Hypersensitivity: etiology
  • Gene expression
  • Humans
  • IncP-1
  • PCR
  • Pesticides
  • Pesticides: metabolism
  • Phylogeny
  • Plasmid
  • Plasmids
  • Plasmids: analysis
  • Plasmids: classification
  • Real-Time Polymerase Chain Reaction
  • Replication
  • Wastewater
  • Water Microbiology

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Authors

  • Alejandro Buschmann

  • Antonia Fortt

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