Effect of estrogen on vascular smooth muscle cells is dependent upon cellular phenotype

  • Song J
  • Wan Y
  • Rolfe B
 et al. 
  • 3


    Mendeley users who have this article in their library.
  • 28


    Citations of this article.


To investigate the growth-regulating action of estrogen on vascular smooth muscle cells (SMC), effects of β-17-estradiol (β-E2) on phenotypic modulation and proliferation of rabbit aortic SMC were observed in vitro. At 10-8M, β-E2significantly slowed the decrease in volume fraction of myofilaments (V(v) myo) of freshly dispersed SMCs in primary culture, indicating an inhibitory effect of β-E2on spontaneous phenotypic modulation of SMC from a contractile to a synthetic phenotype. Freshly dispersed SMCs treated with β-E2also had a relatively longer quiescent phase than control cells before intense proliferation occurred. This was in contrast to SMCs in passage 2-3 (synthetic state), where β-E2-treated cells replicated significantly faster than untreated cells. β-E2also markedly enhanced the serum-induced DNA synthesis of synthetic SMCs in a concentration-dependent manner within physiological range (10-10to 10-8M). These findings indicate that the growth-regulating effect of estrogen on vascular SMC is dependent on the cell's phenotypic state. It delays the cell cycle re-entry of the contractile SMCs by retarding their phenotypic modulation; however, once cells have modulated to the synthetic phenotype, it promotes their replication.

Author-supplied keywords

  • Estradiol
  • Phenotype
  • Proliferation
  • Smooth muscle cell

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Get full text


  • Jian Song

  • Yu Wan

  • Barbara E. Rolfe

  • Julie H. Campbell

  • Gordon R. Campbell

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free