Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows

  • Nsereko V
  • Beauchemin K
  • Morgavi D
 et al. 
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The effects of supplementing a dairy cow diet with incremental levels of a fibrolytic enzyme preparation (preparation B) from Trichoderma longibrachiatum on the rumen microbial population were investigated. Two cows fit- ted with rumen cannulae were each fed a diet containing barley-based concentrate (52%), maize silage (29%), and chopped alfalfa hay (19%), supplemented with 0, 1, 2, 5, or 10 L of preparation B per tonne of dry matter (DM). Preparation B stimulated numbers of total viable bacteria in a quadratic manner (P < 0.05), to approximately 230, 330, 390, and 250% at 1, 2, 5, and 10 L·t–1 DM, respectively. Preparation B increased the numbers of cellobiose-utilizing (P < 0.01), xylanolytic (P < 0.05), and amylolytic bacteria (P < 0.05), but had no effect (P > 0.05) on numbers of cellulolytic bacteria. However, when bacterial numbers enumerated on each substrate were expressed as a proportion of total viable bacterial numbers, only cellobiose utilizers were stimulated, and this stimulation was limited to the 1 L·t–1 DM level of preparation B (P < 0.05). The results of this study demonstrate that the inclusion of an exogenous fibrolytic enzyme preparation in dairy cow diets increased the numbers of rumen bacteria that utilize hemicelluloses and second- ary products of cellulose digestion. Key

Author-supplied keywords

  • cellulase
  • cellulolytic
  • d
  • deux vaches porteuses d
  • diète de doses croissantes
  • fibrolytic enzymes
  • fibrolytique
  • la vache laitière
  • les effets sur la
  • nourries avec un
  • nous avons évalué chez
  • population microbienne du rumen
  • provenant du
  • préparation b
  • rumen
  • rumen ont été chacune
  • résumé
  • soit une préparation enzymatique
  • trichoderma longibrachiatum
  • un ajout à la
  • un supplément
  • une canule reliée au
  • xylanase
  • xylanolytic

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  • V.L. L Nsereko

  • K.A. A Beauchemin

  • D.P. P Morgavi

  • L.M. M Rode

  • A.F. F Furtado

  • T.A. A Mcallister

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