Effect of Nanoparticle Surface on the HPLC Elution Profile of Liposomal Nanoparticles

  • Itoh N
  • Yamamoto E
  • Santa T
 et al. 
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© 2016 Springer Science+Business Media New York Purpose: Nanoparticles have been used in diverse areas, and even broader applications are expected in the future. Since surface modification can influence the configuration and toxicity of nanoparticles, a rapid screening method is important to ensure nanoparticle quality. Methods: We examined the effect of the nanoparticle surface morphology on the HPLC elution profile using two types of 100-nm liposomal nanoparticles (AmBisomeⓇ and DOXILⓇ). Results: These 100-nm-sized nanoparticles eluted before the holdup time (about 4 min), even when a column packed with particles with a relatively large pore size (30 nm) was used. The elution time of the nanoparticles increased with pegylation of the nanoparticles and protein adsorption to the nanoparticles; however, the nanoparticles still eluted before the holdup time. Conclusions: The results of this study indicate that HPLC is a suitable tool for rapid evaluation of the surface of liposomal nanoparticles.

Author-supplied keywords

  • HPLC
  • corona
  • nanomedicine
  • pegylation
  • surface analysis

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