© 2016 Springer Science+Business Media New York Purpose: Nanoparticles have been used in diverse areas, and even broader applications are expected in the future. Since surface modification can influence the configuration and toxicity of nanoparticles, a rapid screening method is important to ensure nanoparticle quality. Methods: We examined the effect of the nanoparticle surface morphology on the HPLC elution profile using two types of 100-nm liposomal nanoparticles (AmBisomeⓇ and DOXILⓇ). Results: These 100-nm-sized nanoparticles eluted before the holdup time (about 4 min), even when a column packed with particles with a relatively large pore size (30 nm) was used. The elution time of the nanoparticles increased with pegylation of the nanoparticles and protein adsorption to the nanoparticles; however, the nanoparticles still eluted before the holdup time. Conclusions: The results of this study indicate that HPLC is a suitable tool for rapid evaluation of the surface of liposomal nanoparticles.
Mendeley saves you time finding and organizing research
Choose a citation style from the tabs below