Effect of nitrite on the immune response of freshwater prawn Macrobrachium malcolmsonii and its susceptibility to Aeromonas hydrophila

  • Chand R
  • Sahoo P
  • 44

    Readers

    Mendeley users who have this article in their library.
  • 27

    Citations

    Citations of this article.

Abstract

The 24- and 96-h lethal concentrations (LC50) of nitrite-N in Macrobrachium malcolmsonii intermoult juveniles were determined and found to be 18.96 and 3.14 mg/l, respectively. Nitrite-N up to 20 mg/l in tryptic soy broth (TSB) had no effect on the growth rate of Aeromonas hydrophila and nitrite-N at 30 mg/l only suppressed growth of the bacteria after 48 h of incubation. Prawns were exposed to 1/10 and 1/50 96 h LC50concentrations of nitrite-N for 120 h. The effects of nitrite stress on the immune ability and disease resistance against experimental A. hydrophila infection in prawns were studied. Parameters measured were total haemocyte count (THC), differential haemocyte count (DHC), haemolymph phenoloxidase (PO) activity, protein content, bacterial agglutinin and haemagglutinin levels. Exposure to nitrite stress significantly reduced PO activity and THC in nitrite exposed prawns compared with values for control prawns. At lower concentration of nitrite stress, total protein content and haemagglutinin levels in haemolymph were decreased compared with values for the control group prawns. DHC and bacterial agglutinin titre were not significantly influenced after 120 h of nitrite stress. However, exposure to higher concentration of nitrite-N to M. malcolmsonii significantly increased the percent mortality with A. hydrophila challenge. In conclusion, nitrite stress reduced the capacity of M. malcolmsonii to resist A. hydrophila infection. © 2006 Elsevier B.V. All rights reserved.

Author-supplied keywords

  • Aeromonas hydrophila
  • Agglutinins
  • Haemocyte count
  • Macrobrachium malcolmsonii
  • Nitrite-N
  • Phenol oxidase

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • R. K. Chand

  • P. K. Sahoo

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free