The effect of speed of deoxygenation on the percentage of aligned hemoglobin in sickle cells. Application of differential polarization microscopy

  • Mickols W
  • Corbett J
  • Maestre M
 et al. 
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Differential polarization microscopy, which provides images of linear dichroism, was used to measure the percentage of aligned Hb in 1086 deoxygenated red blood cells from subjects with sickle cell anemia. The percentage was only slightly dependent on the speed of deoxygenation, thus showing that the percentage of aligned Hb was thermodynamically controlled (as has been found previously for the percentage of polymd. Hb in vitro). A slight decrease in the percentage of aligned Hb due to increasing speed of deoxygenation is primarily due to the increase in the no. of cells contg. no detectable aligned Hb. This class of cells was also the most variable between the different subjects studied. Two other groups of cells that contain different nos. of domains of aligned Hb were identified that contain statistically different percentages of aligned Hb. The differences between these classes of cells was primarily due to different nos. of initial nucleation sites within each cell. Apparently, the presence of preformed nucleation sites within cells at ambient O tensions results in the thermodn. control of aligned Hb polymer. [on SciFinder(R)]

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  • ISSN: 00219258
  • SCOPUS: 2-s2.0-0023888657
  • PUI: 18096614
  • SGR: 0023888657


  • W. E. Mickols

  • J. D. Corbett

  • M. F. Maestre

  • I. Tinoco

  • J. Kropp

  • S. H. Embury

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