Effect of substrate mechanics on chondrocyte adhesion to modified alginate surfaces

  • Genes N
  • Rowley J
  • Mooney D
 et al. 
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Abstract

This study characterized the attachment of chondrocytes to RGD-functionalized alginate by examining the effect of substrate stiffness on cell attachment and morphology. Bovine chondrocytes were added to wells coated with 2% alginate or RGD-alginate. The alginate was crosslinked with divalent cations ranging from 1.25 to 62.5mmol/g alginate. Attachment to RGD-alginate was 10-20 times higher than attachment to unmodified alginate and was significantly inhibited by antibodies to integrin subunits α3and β1, cytochalasin-D, and soluble RGD peptide. The equilibrium level and rate of attachment increased with crosslink density and substrate stiffness. Substrate stiffness also regulated chondrocyte morphology, which changed from a rounded shape with nebulous actin on weaker substrates to a predominantly flat morphology with actin stress fibers on stiffer substrates. The dependence of attachment on integrins and substrate stiffness suggests that chondrocyte integrins may play a role in sensing the mechanical properties of the matrices to which they are attached. © 2003 Elsevier Inc. All rights reserved.

Author-supplied keywords

  • Alginate
  • Attachment
  • Chondrocyte
  • Cytoskeleton
  • Integrin
  • Mechanotransduction

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Authors

  • Nicholas G. Genes

  • Jonathan A. Rowley

  • David J. Mooney

  • Lawrence J. Bonassar

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