Effects of cryopreservation and density-gradient washing on phospholipase C zeta concentrations in human spermatozoa

  • Kashir J
  • Heynen A
  • Jones C
 et al. 
  • 13

    Readers

    Mendeley users who have this article in their library.
  • 26

    Citations

    Citations of this article.

Abstract

Cryopreservation and density-gradient washing (DGW) are routinely used in infertility treatment. This study used quantitative immunofluorescence analysis to report how these techniques affect concentrations of the oocyte activation factor, phospholipase C zeta (PLCζ) in spermatozoa from fertile men. DGW significantly elevated the proportion of spermatozoa in which PLCζ could be detected (by 25-81%; P < 0.0001). In contrast, in four donors, cryopreservation significantly reduced PLCζ concentrations (by 20-56%; P < 0.0001). These findings indicate that while DGW positively selects spermatozoa with detectable PLCζ, cryopreservation has significant detrimental effects upon PLCζ concentrations. Since reduced PLCζ concentrations have been implicated in deficient oocyte activation and infertility, further study is highly warranted. © 2011, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.

Author-supplied keywords

  • assisted reproductive technology (ART)
  • cryopreservation
  • density-gradient washing
  • oocyte activation
  • phospholipase C zeta (PLCζ)
  • spermatozoa

Get free article suggestions today

Mendeley saves you time finding and organizing research

Sign up here
Already have an account ?Sign in

Find this document

Authors

  • Joaquin GadeaUniversidad de Murcia

    Follow
  • Junaid Kashir

  • Ariane Heynen

  • Celine Jones

  • Claire Durrans

  • Jo Craig

Cite this document

Choose a citation style from the tabs below

Save time finding and organizing research with Mendeley

Sign up for free