The effects on newly fertilized eggs, embryos and larvae of zebrafish Danio rerio following exposure to sediment samples from the more heavily contaminated River Korsch, southern Germany, occurred earlier and were more prominent than in samples from the less contaminated Krahenbach. Dose- and time-related effects following exposure to Korsch sediment eluates and extracts included: (1) hatching failure and subsequent death of larvae exposed to undiluted aqueous sediment eluates and reduced hatching rates at sediment extract concentrations greater than or equal to0(.)0125%, (2) increased mortality after exposure to 25 and 50%, dilutions of aqueous sediment eluates, and dilutions of greater than or equal to0(.)00625%, sediment extracts : (3) reduction of heart beat frequency for 50% dilutions of sediment eluates and concentrations to greater than or equal to0(.)025%, extracts (4) increased frequency of heart and yolk sae oedema after exposure to greater than or equal to0(.)0125%, sediment extracts. Since adverse effects of sediment extracts oh cried in zebrafish laboratory, tests correlated with reproductive failure in natural population, of brown trout Salmo trutta f. fario in the severely polluted River Korsch, early life stage tests with zebrafish appear to be a suitable tout to assess the contamination rate Of natural sediment. (C) 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.
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