Efficient FLP recombination in mouse ES cells and oocytes

  • Schaft J
  • Ashery-Padan R
  • Van Hoeven F
 et al. 
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Abstract

We report an improved vector, pCAGGS-FLPe, for transient expression of the enhanced FLP recombinase in mouse ES cells and oocytes. In standard transfection experiments, about 6% of total ES colonies showed FLP recombination, albeit with mosaicism within each colony. After microinjection of pCAGGS-FLPe into oocytes, about one-third of heterozygotic mice born showed complete FLP recombination. Thus pCAGGS-FLPe presents two practical options for removal of FRT cassettes in mice.

Author-supplied keywords

  • Cre
  • Genetic engineering
  • Homologous recombination
  • Site specific recombination
  • Transgenesis

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Authors

  • Julia Schaft

  • Ruth Ashery-Padan

  • Frank Der Van Hoeven

  • Peter Gruss

  • A. Francis Stewart

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